Seonglae ChoSeamless gene editing
CRISPR-Cas12a can induce and track changes in a variety of immune system cells in response to gene editing and fine-tune sets of genes in different directions simultaneously.
New CRISPR tool enables more seamless gene editing — and improved disease modeling
Yale scientists have developed a CRISPR technology that can assess genetic interactions on a host of immunological responses to multiple diseases, including cancer.
https://news.yale.edu/2025/03/20/new-crispr-tool-enables-more-seamless-gene-editing-and-improved-disease-modeling
Simultaneous Editing
Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts
Nature Methods - A single transcript encoding Cas12a and an array of CRISPR RNAs enables multiplexed genome engineering, from multiple knockouts to transcriptional activation or repression to...
https://www.nature.com/articles/s41592-019-0508-6
